Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR)
نویسندگان
چکیده
منابع مشابه
Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR)
Quantitative PCR allows the precise measurement of DNA concentrations and is generally considered to be straightforward and trouble free. However, a survey with 93 validated assays for genes in the Wnt-pathway showed that the amplification of nonspecific products occurs frequently and is unrelated to Cq or PCR efficiency values. Titration experiments showed that the occurrence of low and high m...
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ژورنال
عنوان ژورنال: Biomolecular Detection and Quantification
سال: 2017
ISSN: 2214-7535
DOI: 10.1016/j.bdq.2017.10.001